Enzymatic Incorporation of Emissive Pyrimidine Ribonucleotides

Enzymatic incorporation and utilization of an emissive 6-azauridine.

To display favorable fluorescent properties, the non-emissive native nucleosides need to be modified. Here we present a motif that relies on conjugating 5-membered aromatic heterocycles (e.g., thiophene) to a 6-azapyrimidine (1,2,4-triazine) core. Synthetic accessibility and desirable photophysical properties make these nucleosides attractive candidates for enzymatic incorporation and biochemic...

Divergent prebiotic synthesis of pyrimidine and 8-oxo-purine ribonucleotides

Understanding prebiotic nucleotide synthesis is a long standing challenge thought to be essential to elucidating the origins of life on Earth. Recently, remarkable progress has been made, but to date all proposed syntheses account separately for the pyrimidine and purine ribonucleotides; no divergent synthesis from common precursors has been proposed. Moreover, the prebiotic syntheses of pyrimi...

The ultraviolet absorption spectra of the pyrimidine ribonucleosides and ribonucleotides.

It is well known that the high and characteristic absorption of nucleic acids in ultraviolet light is due to the absorption of their purine and pyrimidine components. Several workers have measured the absorption curves of the aminoand oxypurines (l-3) and Jordan has obtained similar data for adenosine and guanosine and for the corresponding nucleotides (3). In the latter instances substitution ...

Enzymatic Synthesis of Deoxyribonucleic Acid. Iii. the Incorporation of Pyrimidine and Purine Analogues into Deoxyribonucleic Acid.

Enzymatic de novo pyrimidine nucleotide synthesis.

The use of stable isotope labeling has revolutionized NMR studies of nucleic acids, and there is a need for methods of incorporation of specific isotope labels to facilitate specific NMR experiments and applications. Enzymatic synthesis offers an efficient and flexible means to synthesize nucleoside triphosphates from a variety of commercially available specifically labeled precursors, permitti...